Journal: Journal of Thoracic Disease

Article Title: A review of current and novel therapies for idiopathic pulmonary fibrosis

doi: 10.3978/j.issn.2072-1439.2012.12.07

Figure Lengend Snippet: An overview of select clinical trials of in IPF - Unsuccessful Treatments to Date. (Modified from Datta et al., 2011)

Article Snippet: Tralokinumab , Anti-IL-13 antibody; IL-13 stimulates collagen deposition and myofibroblast differentiation , MedImmune LLC. , NCT01629667 , Prospective, double-blinded, randomized placebo-controlled Phase II study; high dose tralokinumab vs. low dose tralokinumab vs. placebo, planned enrollment n=186 , Primary end point: change from baseline in FVC at week 72 Secondary end point: safety , Trial ongoing.

Techniques: Modification, Inhibition, Molecular Weight, Functional Assay, Transformation Assay, Expressing

Journal: Journal of Thoracic Disease

Article Title: A review of current and novel therapies for idiopathic pulmonary fibrosis

doi: 10.3978/j.issn.2072-1439.2012.12.07

Figure Lengend Snippet: An overview of select clinical trials of in IPF - Treatments Under Investigation (Modified from Scotton and Chambers, 2007 and Datta and Scotton, 2011 BJP).

Article Snippet: Tralokinumab , Anti-IL-13 antibody; IL-13 stimulates collagen deposition and myofibroblast differentiation , MedImmune LLC. , NCT01629667 , Prospective, double-blinded, randomized placebo-controlled Phase II study; high dose tralokinumab vs. low dose tralokinumab vs. placebo, planned enrollment n=186 , Primary end point: change from baseline in FVC at week 72 Secondary end point: safety , Trial ongoing.

Techniques: Modification, Expressing, Migration, Inhibition, Activity Assay

Journal: The American Journal of Pathology

Article Title: No Haploinsufficiency but Loss of Heterozygosity for EXT in Multiple Osteochondromas

doi: 10.2353/ajpath.2010.100296

Figure Lengend Snippet: Protein expression of EXT-related genes and HS-dependent pathways in pellets and OCs. Cytoplasmatic NDST1 protein expression in pellets (A) versus nuclear and cytoplasmatic staining in the OCs (L2804; D). SDC2 protein expression is present in the ECM of the pellets (B), while absent in the OCs (L2370; E). SDC3 protein expression is absent in the ECM of the pellets (C) and present in the OCs (L2467; F). Cytoplasmatic CD44v3 protein is predominantly expressed in OCs (dot-like; L2370; J) and weakly in pellets (G). Both BMP (nuclear phosphosmad1) and TGF-β (cytoplasmatic PAI1) signaling is active in the pellets (H and I) and OCs (L2370) (K and L), respectively. Scale bars: 50 μm (A–L); 10 μm (inset J).

Article Snippet: P values <0.05 were considered significant. table ft1 table-wrap mode="anchored" t5 caption a7 Antigen Manufacturer Positive Control Staining Antibody Concentration Antigen Retrieval Collagen II NeoMarkers Growth plate ECM 1 : 100 Proteinase K + hyaluronidase Collagen X Quartett Growth plate ECM 1 : 100 Proteinase K + hyaluronidase SOX9 Atlas Testis Nucleus 1 : 100 Citrate HS chains (10E4) US Biological Skin Membrane; ECM 1 : 400 — Syndecan2 (10H4) David, G. 34 Growth plate Membrane; ECM 1 : 50 — Syndecan3 Proteintech.

Techniques: Expressing, Staining

Journal: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy

Article Title: STAMP2 Attenuates Cardiac Dysfunction and Insulin Resistance in Diabetic Cardiomyopathy via NMRAL1-Mediated NF-κB Inhibition in Type 2 Diabetic Rats

doi: 10.2147/DMSO.S374784

Figure Lengend Snippet: Cardiac fibrosis in control and DM hearts. ( A ): General view of control and DM hearts; ( B and C ): HE and WGA staining in control and DM hearts. The cell cross-sectional area in 8 rats in each group was compared using t tests. ( D ): Collagen I and collagen III immunohistochemistry and Masson staining of control and DM hearts; the data for 8 rats in each group were compared using t tests. **p<0.01 vs control.

Article Snippet: Sections were also incubated with primary antibodies against collagen I (Proteintech, China) and collagen III (Proteintech, China) to measure type I and III collagen deposition.

Techniques: Staining, Immunohistochemistry

Journal: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy

Article Title: STAMP2 Attenuates Cardiac Dysfunction and Insulin Resistance in Diabetic Cardiomyopathy via NMRAL1-Mediated NF-κB Inhibition in Type 2 Diabetic Rats

doi: 10.2147/DMSO.S374784

Figure Lengend Snippet: STAMP2 alleviated myocardial fibrosis in diabetic hearts. ( A ): General view of rat hearts from the control, DM, vehicle and STAMP2 groups; ( B and C ): HE and WGA staining of control, DM, vehicle and STAMP2 group hearts. The cell cross-sectional area in 8 rats from each group was compared using t tests. ( D ): Collagen I and collagen III immunohistochemistry and Masson staining in hearts from rats in the control, DM, vehicle and STAMP2 groups. Data for 8 rats in each group were compared using t tests. *p<0.05 vs vehicle, **p<0.01 vs vehicle, # p<0.05 vs DM, ## p<0.01 vs DM, $$ p<0.01 vs control.

Article Snippet: Sections were also incubated with primary antibodies against collagen I (Proteintech, China) and collagen III (Proteintech, China) to measure type I and III collagen deposition.

Techniques: Staining, Immunohistochemistry